FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l03n01_nutrinetat_vegb18_mh-1_r2o_ln.351000000896dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l03n01_nutrinetat_vegb18_mh-1_r2o_ln.351000000896dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16168937
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1399610.8656165832051915TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG172300.042.8048115
GCACACG176600.042.0867211
CACACGT183050.040.56464812
ACACGTC183600.040.22227513
TCGTATG194150.039.29963745
TCACGTG196300.038.8280430
CACGTGA198050.038.62943631
CGTCTGA191100.038.49390816
AGCACAC204350.038.48214310
AGTCACG199350.038.40366428
ACGTGAA197500.038.39879632
CACGTCT192900.038.28309614
GTCACGT199350.038.13345329
CTCGTAT201950.037.7707344
CGTGAAA201400.037.64323833
GAGCACA212850.036.966319
TCGGAAG211600.036.7482153
AACGATC205050.036.5373638
GATCGGA214200.036.385281
ACGATCT209350.036.02086339
Produced by FastQC (version 0.11.2)