FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l03n01_nutrinetat_vegb16_kn-0_r2o_ln.35100000089656.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l03n01_nutrinetat_vegb16_kn-0_r2o_ln.35100000089656.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23302388
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG2176530.9340373184070234TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG264950.042.97648215
CGTCCGC275100.042.7512733
GCACACG268650.042.69532411
ACGTCCG279100.042.21109432
GTCCGCA283150.041.4710634
CCGCACA283950.041.40100536
CACACGT277800.041.28905512
ACACGTC279550.040.78315413
CGCACAT289700.040.6407637
TCCGCAC292300.040.17278735
GTCACGT294950.039.9593129
CACGTCT287450.039.67889814
TCGTATG298850.039.5157345
AGTCACG299750.039.44732328
AGCACAC312650.038.66875510
CGTCTGA298350.038.18129316
CTCGTAT311000.037.9433144
GCACATC310600.037.93483438
TCACGTC311600.037.9098830
CACGTCC314200.037.62315431
Produced by FastQC (version 0.11.2)