FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l03n01_nutrinetat_vegb14_ge-0_r2o_ln.351000000895dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l03n01_nutrinetat_vegb14_ge-0_r2o_ln.351000000895dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22655974
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG2008510.886525558336181TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG235700.043.1732315
GCACACG240050.042.69906211
ACACGTC245650.041.38564713
CACACGT252700.040.59935412
CACGTCT255150.039.92891314
TCGTATG270400.039.3303245
TATCTCG273950.038.77189641
AGCACAC284550.038.3916210
CGTCTGA267050.038.1585816
CTCGTAT282000.037.6651144
GAGCACA296400.036.9468659
TCGGAAG300450.036.1863863
CAGTCAC298650.035.89141527
GATCGGA307350.035.4490241
TCTCGTA301600.035.40929843
CCTTTAT300850.035.30517237
AGAGCAC311050.035.1208348
ATCGGAA312600.034.8084032
GTCACAT308200.034.57522229
ACTCCAG315250.034.4157823
Produced by FastQC (version 0.11.2)