FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l03n01_nutrinetat_vegb12_edi-0_r2o_ln.35100000089570.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l03n01_nutrinetat_vegb12_edi-0_r2o_ln.35100000089570.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15979204
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC1036820.6488558503915465TruSeq Adapter, Index 16 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG129450.041.32026715
GCACACG131700.040.72302611
CCCGTCC134550.040.6406733
CACCCGT138450.039.85027731
TCACCCG140300.039.16758730
ACACGTC137500.038.98790713
CACACGT138550.038.9335112
CGTATGC141350.038.76248644
ACCCGTC142400.038.6041632
GTATGCC142950.038.468845
GTCACCC144100.038.2274229
TCGTATG146300.037.5422843
CCGTCCA147400.037.00546334
CACGTCT145300.036.84569514
CGTCTGA145900.036.6124116
AGCACAC156200.036.4313810
CTCGTAT153400.035.8191842
TCGGAAG163800.034.6112943
GAGCACA165500.034.5052539
GATCGGA166200.034.197431
Produced by FastQC (version 0.11.2)