FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l03n01_nutrinetat_vegb10_ct-1_r2o_ln.351000000894f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l03n01_nutrinetat_vegb10_ct-1_r2o_ln.351000000894f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37157062
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG3733551.004802263429762TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG450050.043.4799915
GCACACG458700.042.91488611
CACACGT468500.042.0884812
ACACGTC467150.041.9803213
CACGTCT483000.040.60764714
TCGTATG508250.039.56973345
CTCGTAT513950.039.2045844
AGCACAC530350.039.13406810
CGTCTGA503500.038.878516
ATGTCAG528750.038.22101234
ACATGTC534650.037.94311532
TGTCAGA534400.037.8335335
TCGGAAG545800.037.783063
CACATGT538700.037.7653231
GTCAGAA535500.037.6351436
GAGCACA554700.037.492449
CAGTCAC550850.036.9794427
GATCGGA561050.036.854281
TCTCGTA550100.036.78203243
ATCGGAA566900.036.4081952
Produced by FastQC (version 0.11.2)