FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l02n01_nutrinetat_vegb35_tsu-0_r2y_ln.3510000008963c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l02n01_nutrinetat_vegb35_tsu-0_r2y_ln.3510000008963c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34177288
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG4344131.2710575514359126TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG502900.041.6421215
GCACACG510050.041.24386211
ACGTGAA506700.040.7111732
GTCACGT506200.040.6551929
CACACGT518200.040.5602312
CTCGTAT507350.040.4478244
TCGTATG511700.040.1655245
AGCACAC526450.040.0830710
CACGTGA515550.040.00818631
AGTCACG517750.040.00034728
ACACGTC524900.039.9996513
TCACGTG516300.039.8719130
CACGTCT531850.039.4473414
AACGATC520550.039.10655638
CGTGAAA528050.039.01292433
TCGGAAG547250.038.5541233
TCTCGTA533900.038.49540343
GAGCACA552200.038.315789
GATCGGA551800.038.1834681
ACGATCT533800.038.1527139
Produced by FastQC (version 0.11.2)