FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l02n01_nutrinetat_vegb33_stw-0_r2y_ln.351000000895b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l02n01_nutrinetat_vegb33_stw-0_r2y_ln.351000000895b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22515708
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG3609721.6032007521149236TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC385150.043.57833533
GTCCGCA390300.042.92340534
ACGTCCG391750.042.8918132
CCGCACA392000.042.72592536
CGCACAT397500.042.14606537
ACGTCTG406100.042.1066415
GCACACG409250.041.88148511
TCCGCAC400050.041.77953335
GTCACGT404750.041.5265129
TCGTATG407100.041.23536745
CACACGT417400.041.0583412
CTCGTAT409200.041.05673644
AGTCACG411750.040.93530328
ACACGTC421100.040.6815513
AGCACAC421850.040.6786410
GCACATC415400.040.28671638
TCACGTC418600.040.1733330
CACGTCT428350.039.9772414
TCGGAAG430150.039.870673
GAGCACA432450.039.754379
Produced by FastQC (version 0.11.2)