FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l02n01_nutrinetat_vegb31_st-0_r2y_ln.3510000008953d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l02n01_nutrinetat_vegb31_st-0_r2y_ln.3510000008953d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21271923
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG2610531.2272186205262212TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG309600.041.47599415
GCACACG316600.040.8078511
TCGTATG312550.040.3866345
AGCACAC324000.039.9661810
TATCTCG316400.039.9449741
ACACGTC324100.039.80085813
CACACGT325300.039.70936212
CACGTCT326950.039.42639514
CTCGTAT321850.039.1916844
TCGGAAG330650.038.8523333
GATCGGA335750.038.2889181
GAGCACA340550.037.9974829
CGTCTGA339250.037.83778416
ATCGGAA344000.037.4426462
CAGTCAC339800.037.4162627
TCTCGTA341950.036.99323743
CGGAAGA352050.036.5418324
GTCACAT347050.036.52473429
CCTTTAT348150.036.29541837
AGAGCAC357200.036.238928
Produced by FastQC (version 0.11.2)