FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l02n01_nutrinetat_vegb29_shahdara_r2y_ln.351000000894a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l02n01_nutrinetat_vegb29_shahdara_r2y_ln.351000000894a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20175526
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC2120201.0508771865477016TruSeq Adapter, Index 16 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC242600.041.7484133
CACCCGT245900.041.4460131
ACGTCTG256400.040.983215
GTATGCC248650.040.91281545
TCACCCG252150.040.41909830
ACCCGTC252200.040.3747932
GCACACG261250.040.35153211
CGTATGC254100.040.07957544
CACACGT264200.039.85840612
ACACGTC266150.039.56637213
TCGTATG259400.039.27802743
GTCACCC260400.039.260329
AGCACAC269950.039.1094110
CTCGTAT262350.038.79348842
CACGTCT271750.038.759314
CCGTCCA263700.038.33440834
GAGCACA276950.038.15349
CGTCTGA276550.038.07842616
TCGGAAG281950.037.3439373
GATCGGA290050.036.4716761
Produced by FastQC (version 0.11.2)