FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l02n01_nutrinetat_vegb27_sakata_r2y_ln.35100000089421.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l02n01_nutrinetat_vegb27_sakata_r2y_ln.35100000089421.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13738767
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1699121.2367339805675428TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG203100.041.060811
ACGTCTG202650.041.00766415
TCGTATG201250.040.19759845
ACACGTC208450.040.02853413
AGCACAC208700.039.9913610
CTCGTAT203950.039.69853244
CACACGT210450.039.60536212
GAGCACA214900.038.9841429
CACGTCT213550.038.96722414
TCGGAAG216650.038.505773
ACATGTC210800.038.50454332
TGTCAGA210100.038.45278535
ATGTCAG210450.038.41245334
GTCAGAA211200.038.122636
GATCGGA220350.038.018071
CGTCTGA218750.037.97921416
TCTCGTA213950.037.90611643
CACATGT214550.037.75812531
CAGTCAC218400.037.2470727
ATCGGAA224900.037.20332
Produced by FastQC (version 0.11.2)