FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l02n01_nutrinetat_vegb25_oy-0_r2y_ln.351000000893a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l02n01_nutrinetat_vegb25_oy-0_r2y_ln.351000000893a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7090094
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCC487600.6877200781823203TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATCG58000.041.0071233
ACGTCTG61500.039.4699615
GCACACG62300.039.10756711
GTATGCC60950.039.01410745
ACTAATC62050.038.4401932
CTCGTAT63050.037.75034742
CGTATGC63300.037.63679544
CACACGT64750.037.5930812
TCGTATG63700.037.50640543
CACTAAT64250.037.43941531
AGCACAC65100.037.4255210
ACACGTC65000.037.41387613
AGTCACT65800.036.6261428
CACGTCT68750.035.4058414
GAGCACA69450.035.308129
GATCGGA70050.034.4403271
TCTCGTA69400.034.36107641
CAGTCAC70850.034.07808327
TCGGAAG71100.033.8904233
CGTCTGA72800.033.3434416
Produced by FastQC (version 0.11.2)