FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l02n01_nutrinetat_vegb23_n13_r2y_ln.35100000089322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l02n01_nutrinetat_vegb23_n13_r2y_ln.35100000089322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17112604
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1747301.0210602664562332TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG208400.040.84724411
ACGTCTG211650.040.26241315
CACACGT213300.039.88768412
AGCACAC215100.039.6167610
CTCGTAT210250.039.13978244
TCGTATG210500.039.1360445
ACACGTC219150.038.7921213
CACGTCT222750.038.22577314
GAGCACA228300.037.473989
CACAGTC220000.037.4373331
ACAGTCA220300.037.3248432
TCGGAAG228650.037.2037543
GATCGGA230150.037.0842931
GTCACAG222850.037.05040429
TCTCGTA224800.036.68654643
CAGTCAC229200.036.356927
ATCGGAA234550.036.287092
CGTCTGA237950.035.83122316
TCACAGT231850.035.44644530
CTGAACT241050.035.2417219
Produced by FastQC (version 0.11.2)