FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l02n01_nutrinetat_vegb21_mt-0_r2y_ln.351000000892ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l02n01_nutrinetat_vegb21_mt-0_r2y_ln.351000000892ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25293264
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2827711.1179695906388356TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG325850.041.43004615
GTATGCC321600.041.3901345
GCACACG333150.040.67094811
CGTATGC327800.040.56609744
TCGTATG330350.040.28701443
ACACGTC344600.039.25413513
CACACGT345500.039.21699512
CTCGTAT338600.039.19911642
AGCACAC349400.038.76024210
CACGTCT356200.037.9884214
GAGCACA357500.037.9764339
GTCACCT352300.037.89169329
TCGGAAG357100.037.8141443
CGTCTGA357850.037.7629716
GATCGGA362800.037.2755051
ATCGGAA367700.036.730162
TCTCGTA364400.036.54106541
CAGTCAC372650.035.94231827
CGGAAGA378100.035.797364
AGAGCAC381800.035.582968
Produced by FastQC (version 0.11.2)