FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l02n01_nutrinetat_vegb19_mr-0_r2y_ln.351000000892c3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l02n01_nutrinetat_vegb19_mr-0_r2y_ln.351000000892c3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35615374
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC2491900.6996697549771623TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC305300.039.6578245
ACGTCTG315400.039.48629415
CGGCTAC309150.039.34836633
CGTATGC308950.039.08735744
ACGGCTA314800.038.8222132
GCACACG323350.038.62689611
GGCTACA316000.038.3835834
AGTCACG320850.038.16153728
TCGTATG319200.037.99429743
CACACGT334150.037.3581412
GTCACGG329500.037.13923629
CACGGCT328150.037.12671331
ACACGTC337150.036.96566813
GCTACAT332250.036.47062735
AGCACAC344100.036.382710
TCACGGC337300.036.206430
CTCGTAT337800.035.85563342
CTACATC343800.035.2495136
CACGTCT354050.035.17576214
TCGGAAG357950.034.977713
Produced by FastQC (version 0.11.2)