FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l01n01_nutrinetat_vegb9_ct-1_r2y_ln.3510000008954a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l01n01_nutrinetat_vegb9_ct-1_r2y_ln.3510000008954a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30616169
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG4689461.5316939229072064TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG569700.042.20648215
GCACACG577950.041.91540511
CACACGT585800.041.3191612
TCGTATG553900.041.2036745
ACACGTC589950.041.0208713
AGCACAC591450.041.00432210
CTCGTAT558350.040.9976544
CACGTCT596200.040.31161514
ATGTCAG587150.040.0165134
CACATGT589250.039.98570331
GAGCACA609550.039.8900879
TGTCAGA589300.039.79014235
ACATGTC591900.039.75255632
TCGGAAG597600.039.618793
GTCAGAA593750.039.42403836
CGTCTGA611750.039.3016516
TCTCGTA587000.039.1654243
GATCGGA609650.038.9060821
CAGTCAC610250.038.8382627
CATGTCA609900.038.60992433
Produced by FastQC (version 0.11.2)