FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l01n01_nutrinetat_vegb7_col-0_r2y_ln.351000000894eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l01n01_nutrinetat_vegb7_col-0_r2y_ln.351000000894eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27639501
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGCC2111950.764105690620102TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC251200.040.733845
CTAATCG251800.040.65310333
ACGTCTG263850.039.59723315
GCACACG268700.039.29248411
ACTAATC261850.039.18492532
CGTATGC265350.038.4828944
CACACGT275100.038.3538412
ACACGTC275950.038.19514513
CTCGTAT270200.037.82701542
AGCACAC280500.037.6555810
CACTAAT275150.037.60172731
AGTCACT278350.037.0397728
TCGTATG276300.036.98267743
CACGTCT284000.036.85907714
TCGGAAG288900.035.754473
GAGCACA304300.034.9100659
TCTCGTA294200.034.7503541
GATCGGA297500.034.6815261
CGTCTGA303850.034.39190716
CAGTCAC302650.034.1476627
Produced by FastQC (version 0.11.2)