FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l01n01_nutrinetat_vegb5_bur-0_r2y_ln.35100000089458.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l01n01_nutrinetat_vegb5_bur-0_r2y_ln.35100000089458.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24143091
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG3745651.551437634891075TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG447950.042.01651815
GCACACG455350.041.6942811
ACACGTC460400.041.11487213
AGCACAC461750.041.09202610
CACACGT463350.040.94527412
TCGTATG448950.040.88273245
CTCGTAT450250.040.65765844
CACGTCT463300.040.64385214
GAGCACA477700.039.7435469
GTCACAG465600.039.56040229
CACAGTC465250.039.42613631
ACAGTCA467050.039.20625332
TCTCGTA467150.039.182343
TCGGAAG472350.039.020583
CAGTCAC475650.038.9562527
CGTCTGA483500.038.94581216
GATCGGA482150.038.3789061
AATCTCG478550.038.260641
TCCAGTC489400.038.1503725
CTGAACT493450.038.09667219
Produced by FastQC (version 0.11.2)