FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l01n01_nutrinetat_vegb3_bi-1_r2y_ln.351000000893df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l01n01_nutrinetat_vegb3_bi-1_r2y_ln.351000000893df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27891100
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2646710.9489442868872149TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC310850.041.06742545
ACGTCTG319950.040.9651615
GCACACG328200.040.12734211
CGTATGC318850.039.92825744
ACACGTC333800.039.46762513
CACACGT334250.039.36064512
CTCGTAT327800.038.98970442
AGCACAC338750.038.93075610
TCGTATG328650.038.8197843
CACGTCT346400.037.91512714
GTCACCT341550.037.757429
TCGGAAG347450.036.8330083
GAGCACA360650.036.6790169
CGTCTGA363200.036.1737316
TCTCGTA355400.036.0261441
GATCGGA359400.035.8004261
CAGTCAC363000.035.6008627
ATCGGAA361450.035.512182
CTGAACT377100.034.7508719
AATCTCG368200.034.75066439
Produced by FastQC (version 0.11.2)