FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l01n01_nutrinetat_vegb1_akita_r2y_ln.35100000089359.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l01n01_nutrinetat_vegb1_akita_r2y_ln.35100000089359.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27065269
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC2742691.0133614411887057TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC325400.041.8100733
GTATGCC326950.041.39245
ACGTCTG343100.041.09920515
ACGGCTA335300.040.748532
GGCTACA333350.040.61778634
GCACACG350200.040.59993411
CGTATGC333900.040.35196344
CACACGT355100.040.0460312
AGTCACG344500.039.81089828
CTCGTAT339400.039.6861942
ACACGTC357650.039.6599413
AGCACAC359100.039.6250210
GTCACGG347600.039.4170129
TCACGGC348550.039.17430
TCGTATG345450.039.0160543
CACGTCT365400.038.6217514
GCTACAT351700.038.51098335
CACGGCT355850.038.35154731
GAGCACA375500.037.9962439
CTACATC357300.037.95182436
Produced by FastQC (version 0.11.2)