FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l01n01_nutrinetat_vegb17_mh-1_r2y_ln.35100000089748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l01n01_nutrinetat_vegb17_mh-1_r2y_ln.35100000089748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21951721
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG2860941.3032873367878537TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG342700.041.8563715
GTCACGT341500.041.1946429
GCACACG352450.041.06231311
TCGTATG343950.040.7979645
ACGTGAA346100.040.76944432
AGTCACG347600.040.7306528
CACACGT355800.040.65672312
TCACGTG346500.040.59370430
CACGTGA348450.040.46994831
ACACGTC358400.040.3429513
AGCACAC360950.040.14519510
CTCGTAT349750.040.0479944
CACGTCT362800.039.5870314
AACGATC350300.039.5070438
CGTGAAA357700.039.3543933
GAGCACA371950.039.084979
TCGGAAG362150.038.8554653
CGTCTGA370650.038.6879216
GATCGGA367900.038.3057371
ACGATCT362000.038.248839
Produced by FastQC (version 0.11.2)