FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l01n01_nutrinetat_vegb15_kn-0_r2y_ln.351000000896cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l01n01_nutrinetat_vegb15_kn-0_r2y_ln.351000000896cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27392146
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG3632251.3260187792515417TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC412200.043.32777833
ACGTCCG419350.042.68351432
GTCCGCA421800.042.2515934
CCGCACA422750.042.16744636
CGCACAT424700.041.97913437
ACGTCTG435600.041.8236115
GCACACG444750.041.38300311
GTCACGT436200.041.1131929
TCCGCAC436150.040.7991835
AGTCACG443050.040.726428
TCGTATG440600.040.57042345
CACACGT454050.040.4858412
AGCACAC455700.040.4281110
ACACGTC454700.040.40817313
CTCGTAT445800.040.03407344
CACGTCT458900.039.7736114
TCACGTC451700.039.67251230
GAGCACA471450.039.1824879
GCACATC454650.039.16434538
TCGGAAG462000.038.8875473
Produced by FastQC (version 0.11.2)