FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l01n01_nutrinetat_vegb13_ge-0_r2y_ln.35100000089649.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l01n01_nutrinetat_vegb13_ge-0_r2y_ln.35100000089649.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28245890
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG4230241.497647976395858TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG498450.042.15978615
GCACACG507100.041.8042711
TATCTCG499850.041.18548641
CACACGT515200.041.12082312
AGCACAC516650.041.07073610
TCGTATG502250.041.0104245
ACACGTC518800.040.81394613
CTCGTAT506700.040.6074944
CACGTCT521450.040.32609614
GAGCACA537200.039.6210639
TCGGAAG527150.039.1470953
GATCGGA530650.038.9327851
CGTCTGA540050.038.9038916
TCTCGTA530150.038.7945443
CAGTCAC538050.038.62096827
CCTTTAT537450.038.2886237
ATCGGAA542300.038.0907972
CTGAACT554200.037.8699919
AGAGCAC565800.037.673978
ATCTCGT545600.037.66831642
Produced by FastQC (version 0.11.2)