FastQCFastQC Report
Sun 16 Apr 2017
C8PYJACXX_l01n01_nutrinetat_vegb11_edi-0_r2y_ln.351000000895c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8PYJACXX_l01n01_nutrinetat_vegb11_edi-0_r2y_ln.351000000895c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27914336
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCC2785470.9978636067144853TruSeq Adapter, Index 16 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC327700.041.83245533
GTATGCC332250.041.02880545
CACCCGT336450.040.8430231
ACGTCTG348650.040.6323615
TCACCCG337950.040.6149130
GCACACG355550.040.19816211
CGTATGC340000.039.98556544
ACCCGTC346800.039.70166832
GTCACCC348650.039.542729
CACACGT361900.039.43688212
AGCACAC364150.039.33530410
ACACGTC365150.039.12284513
TCGTATG348450.039.0808243
CTCGTAT353500.038.54908442
CACGTCT372000.038.10610614
CCGTCCA364150.037.49197834
GAGCACA385600.037.26979
GATCGGA378150.036.846081
TCGGAAG377450.036.8207663
CGTCTGA386000.036.74732616
Produced by FastQC (version 0.11.2)