FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_vs7838-t4input-160321.351000000578e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_vs7838-t4input-160321.351000000578e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19291062
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG688420.3568595653261598TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG89650.037.44566711
ACACGTC95650.035.1908413
GATCGGA97550.035.0521851
CACACGT95900.035.05217712
ACGTCTG95950.034.86967515
CACGTCT98050.034.05400514
CGTCTGA100550.033.49786816
AGCACAC101650.033.37928410
TCCAGTC105200.031.58904825
GAACTCC106000.031.58412621
CACAGTC103800.031.5169731
GAGCACA108700.031.2760819
CAGTCAC106150.031.09438327
GTCACAG107750.030.67481429
AGAGCAC112100.030.4475888
CCAGTCA109300.030.36292626
AGTCACA110200.029.89036828
ATCTCGT110750.029.5807142
TCGGAAG116250.029.5734623
CTCGTAT112500.029.14116944
Produced by FastQC (version 0.11.2)