FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_vs7838-t4hop1-160321.35100000057833.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_vs7838-t4hop1-160321.35100000057833.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17751765
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC504610.2842590581837919TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG67800.036.3715711
ACACGTC71600.034.5983613
GATCGGA73700.034.1781161
CACACGT73000.033.8423612
ACGTCTG74450.033.6666915
CACGTCT74300.033.46211614
CGTCTGA76900.032.5061316
CATCTCG75350.031.98029939
AGCACAC78800.031.52274510
CAGTCAC80650.030.63166427
CGTATGC81250.030.129944
GAGCACA83400.029.6758339
CCAGTCA83550.029.59537726
AGTCACC83300.029.30605728
GTATGCC84250.029.16475745
TCGGAAG87450.029.0730953
TCCAGTC85200.029.02222425
GAACTCC86050.028.86636221
CTCGTAT85600.028.54587642
AGAGCAC87800.028.265388
Produced by FastQC (version 0.11.2)