FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_vs7813-t4input-160321.3510000005795c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_vs7813-t4input-160321.3510000005795c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22289742
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG3968891.7805903720195595TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG459700.043.0080111
GATCGGA467050.042.9105221
ACACGTC464550.042.55899413
CACACGT465350.042.51484312
ACGTCTG467450.042.24683415
CACGTCT467400.042.198414
AGCACAC473000.041.9842110
CGTCTGA470900.041.93253716
GAGCACA477050.041.683359
AGAGCAC479650.041.4386378
TCGGAAG486300.041.158833
TATCTCG467400.041.143741
CAGTCAC475850.041.1314227
AGTTCCG472000.041.10482434
CCGTATC467600.041.1019538
TCCAGTC478550.040.93217525
GTTCCGT473100.040.89986835
GAACTCC483300.040.77662321
CCAGTCA480050.040.7434426
GTCACAG479350.040.72350729
Produced by FastQC (version 0.11.2)