FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_vs7813-t4hop1-160321.35100000057876.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_vs7813-t4hop1-160321.35100000057876.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16429597
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC7061794.2982125489748775TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG779250.044.19763611
GATCGGA790250.044.0511551
ACACGTC781400.044.01268413
ACGTCTG785150.043.91982715
CACACGT784200.043.88422812
CACGTCT785300.043.81975614
AGCACAC788800.043.72243510
CGTCTGA789050.043.6826516
GAGCACA793350.043.513039
TCGGAAG801950.043.3912283
AGAGCAC798450.043.308238
TCCAGTC792150.043.1441225
GAACTCC796100.043.14214721
GTATGCC783700.043.1300145
CCAGTCA794450.042.9625726
CAGTCAC794950.042.9581927
ATCGGAA811450.042.9303672
CGTATGC788000.042.9138644
AGTCACC796650.042.7874428
GTCACCT796900.042.77184729
Produced by FastQC (version 0.11.2)