FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_rs-wtinput-160321.35100000057ec0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_rs-wtinput-160321.35100000057ec0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21796882
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1694310.7773175998291866TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC199150.041.6892229
GCACACG203400.041.3274511
TCACGCC203500.040.5990630
AGTCACG207300.040.23435228
CACACGT210100.040.00953712
CACGCCA207950.039.73026331
ACACGTC212250.039.6148613
ACGTCTG213750.039.2945715
CACGTCT213650.039.28137214
GATCGGA216750.039.1847151
AGCACAC216250.039.00695410
TATCTCG214800.038.62020539
GAGCACA221900.038.2059759
CGTCTGA221700.037.95636716
GTATGCC219950.037.8308945
CAGTCAC221300.037.75002727
GAACTCC223550.037.41033621
TCCAGTC225050.037.14099525
CGTATGC224050.037.12753744
CCAGTCA226200.036.85270326
Produced by FastQC (version 0.11.2)