FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_rs-wtchip-160321.35100000057a31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_rs-wtchip-160321.35100000057a31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15602349
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC8955155.739616515436233TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG973700.044.33901211
ACACGTC977200.044.1571813
CACACGT978200.044.1350412
GATCGGA989550.044.1079031
CACGTCT977800.044.06795514
AGCACAC983150.043.96088410
ACGTCTG981400.043.89254815
GAGCACA987850.043.8190779
CGTCTGA986800.043.61815616
TCGGAAG999300.043.5985763
AGAGCAC997500.043.4538088
GTATGCC969200.043.43538745
CAGTCAC984550.043.3809227
ATCGGAA1007050.043.3658262
GAACTCC989350.043.3546621
CGGAAGA1006900.043.260564
TCCAGTC986700.043.2407925
GTCTGAA996500.043.23776217
ACTGACC986500.043.19136432
CCAGTCA988500.043.15067326
Produced by FastQC (version 0.11.2)