FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_rs-hainput-160321.35100000057ab7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_rs-hainput-160321.35100000057ab7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24564021
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC2883391.1738265490002635TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG329700.042.40014611
CACACGT336500.041.56337712
ACACGTC337450.041.3930313
GTCACAC334300.041.32488329
ACGTCTG338000.041.26576215
GATCGGA344200.041.1155241
CACGTCT340000.041.0627214
AGCACAC344500.040.69616310
CGTCTGA345350.040.4261116
GATCTCG340050.040.3875839
CACACAG342450.040.35452731
CAGTCAC346600.039.86420427
GAGCACA353350.039.7526479
GTATGCC348350.039.69308545
GAACTCC349350.039.6856521
TCCAGTC351900.039.25101525
CCAGTCA351650.039.2469326
AGTCACA353850.039.1237328
CACAGTG352900.039.07029333
AGAGCAC361300.038.90868
Produced by FastQC (version 0.11.2)