FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_rs-hachip-160321.351000000579c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_rs-hachip-160321.351000000579c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16443652
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1021210.6210360083027785TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG119800.041.13138211
ACACGTC123250.039.99829513
CACACGT123400.039.8949712
ACGTCTG128000.038.6370315
CACGTCT127700.038.6220814
GATCGGA129450.038.5332831
AGCACAC129400.038.14945610
TATCTCG127750.037.91914739
CCGATGT129550.037.37537433
CAGTCAC130800.037.29263327
CGTCTGA133350.037.06947716
GAGCACA134450.036.983639
TCCAGTC134100.036.45880525
AGTCACC135050.036.1023828
GAACTCC135950.036.09507421
GTATGCC135050.036.0896745
CACCGAT135200.036.02980831
CGATGTA134650.036.00987634
CCAGTCA137150.035.7136426
GTCACCG136700.035.63445329
Produced by FastQC (version 0.11.2)