FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_mj-83c_160531.35100000057e57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_mj-83c_160531.35100000057e57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8577585
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG277220.32319120125303336TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG35150.036.2318311
GATCGGA37400.034.246971
CACACGT37800.033.87034612
GTCACAC36750.033.79544429
ACACGTC38450.033.18072513
ACGTCTG38800.033.055215
CACGTCT39850.032.0713114
AGCACAC39950.032.04753510
CGTCTGA40200.032.01540816
TATCTCG39800.031.71419141
GAGCACA41000.031.4997379
CACACTG39700.031.45421631
TCCAGTC42650.030.01663825
GAACTCC43200.029.68656221
CAGTCAC42800.029.64859627
CTCGTAT43100.029.18342244
AGAGCAC43750.029.1592488
AGTCACA43300.028.94250328
TCACACT43850.028.68260630
ATCTCGT44550.028.43427342
Produced by FastQC (version 0.11.2)