FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_mj-160-160531.35100000057deb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_mj-160-160531.35100000057deb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7543623
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG149040.19757084891437443TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG19900.033.9192733
GCACACG21050.033.02883511
GATCGGA22950.030.7974871
ACGTCTG23350.029.96818515
ACACGTC23450.029.74443813
CGTACGT22900.029.2791934
CACGTCT24500.028.3778414
CACACGT24850.027.97815112
CGTCTGA25150.027.6442316
AGCACAC26350.026.98319610
GTACGTA25700.026.35188335
GAGCACA27200.026.0565599
GAACTCC27200.025.72551221
CAGTCAC27500.025.03578827
CCAGTCA28200.024.81326126
ACCGTAC27650.024.6561832
AGTCACC28600.024.23021328
GTCACCG28650.024.03118129
CTCGTAT29000.023.58722944
TCCAGTC29600.023.41162525
Produced by FastQC (version 0.11.2)