FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_mj-150_160531.35100000057baa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_mj-150_160531.35100000057baa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6766410
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC85860.12689151263373044TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG14150.031.32470311
CTTAGGC14600.028.97224633
GATCGGA15650.028.4804131
CATCTCG15600.028.26888339
ACACGTC15900.028.01852413
ACTTAGG15450.027.66956132
CACACGT16600.027.10809912
ACGTCTG16150.026.74889815
CACGTCT16800.026.24967614
CGTCTGA16950.026.01737816
AGCACAC17800.025.53339410
GAGCACA18800.023.816029
GCATCTC19650.022.78631638
GAACTCC19100.022.49955621
GTATGCC20050.022.22098445
CAGTCAC19800.022.04485727
TAGGCAT19600.022.04054535
ATCTCGT20700.021.30440140
AGTCACT20700.021.3037728
CCAGTCA21000.021.1065726
Produced by FastQC (version 0.11.2)