FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l08n01_mj-147-160531.35100000057b23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l08n01_mj-147-160531.35100000057b23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9132342
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC508170.556450908211716TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG59250.040.59450511
ACACGTC62750.038.50955613
CACACGT63250.038.0984112
GATCGGA64150.037.8972051
CGATCTC62750.037.54143538
CACGATC63500.037.41692736
CACGTCT64900.037.40715414
ACGTCTG64850.037.3319115
GATCTCG64350.036.8175939
AGCACAC66000.036.6473410
CATCACG65300.036.5235533
CGTCTGA66800.036.3431816
GAGCACA67200.035.9590459
GAACTCC67750.035.33503321
GTATGCC68350.035.2252545
ATCACGA68150.034.89710634
CCAGTCA68600.034.7004226
TCACGAT68550.034.69347835
CTCGTAT69450.034.18002742
TCCAGTC69650.034.08038725
Produced by FastQC (version 0.11.2)