FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_vs6639-t6sir3-150818.3510000005785d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_vs6639-t6sir3-150818.3510000005785d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22597716
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG2029050.8979004780837143TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG256100.043.43487535
GTCCCGA247850.042.9198536
ACCCGTC270950.042.3083132
ACACGTC276800.042.26015513
CACGTCT277600.042.09765214
CACCCGT273400.041.98678231
TCACCCG274000.041.96874230
ACGTCTG280100.041.68175515
CCCGATC252050.041.50912538
AGCACAC283550.041.35802510
TCCCGAT254800.041.21051437
GATCGGA286650.041.207371
CGATCTC254700.040.88299640
CGTCTGA286300.040.8341216
CCGATCT255850.040.7519139
GAACTCC286200.040.60414521
CACACGT289500.040.421812
GAGCACA290900.040.4053349
CCCGTCC283400.040.39400533
GATCTCG253000.040.3751941
Produced by FastQC (version 0.11.2)