FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_vs6639-t6input-150818.351000000578fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_vs6639-t6input-150818.351000000578fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15012861
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1031330.6869643301166912TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA133250.040.1000441
GCACACG132550.039.8733511
AGTCACG132450.039.3083728
ACACGTC134650.039.13451813
CACACGT135550.039.02406712
ACGTCTG135700.038.68248415
CACGTCT137500.038.2251814
CGATCTC133650.037.99598340
GATCTCG136400.037.72479641
CGTCTGA139100.037.70462416
GTCACGT137600.037.69000229
AGCACAC141500.037.47878310
CACGTGA139650.037.233431
TCACGTG139550.037.14721730
CAGTCAC141350.036.91293727
GAGCACA144150.036.8985569
TCCAGTC141550.036.78130325
GAACTCC142300.036.55582421
CCAGTCA143650.036.21227626
TCGGAAG148250.036.0934523
Produced by FastQC (version 0.11.2)