FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_vs6639-t6hop1-150818.351000000577ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_vs6639-t6hop1-150818.351000000577ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18755363
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2207341.1769113719633153TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG253650.042.5606711
ACACGTC258750.041.7391813
GATCGGA262600.041.6122471
CACACGT260000.041.59043512
CACGTCT261350.041.25485214
ACGTCTG261000.041.24983215
AGCACAC264700.040.86089310
CGTCTGA263800.040.77788516
CCGTATC261350.040.3677938
GAGCACA268450.040.2974139
TATCTCG264350.039.96074341
AGTTCCG265050.039.9393534
CAGTCAC269350.039.71950527
GAACTCC270950.039.6509321
TCGGAAG275250.039.6241573
GTTCCGT267150.039.549635
TCCAGTC270650.039.51199325
AGAGCAC274350.039.4636088
GTCACAG270900.039.45902629
CCAGTCA272600.039.25421526
Produced by FastQC (version 0.11.2)