FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_vs6639-t3sir3-150818.3510000005780c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_vs6639-t3sir3-150818.3510000005780c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19028875
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG1354620.7118760305062701TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG161800.041.4115815
ACACGTC161950.041.3735513
CACGTCT162450.041.2044414
GATCGGA169500.040.247941
AGCACAC168000.040.2455310
CGTCTGA166550.040.14946716
GAACTCC167550.039.77503221
GTCTGAA173450.038.69456517
GAGCACA175450.038.6900989
TCCAGTC171600.038.626525
CCAGTCA172250.038.46777726
CAGTCAC172600.038.38977427
AGTCACA174600.038.16909428
CACACGT176750.038.1636712
ATCTCGT173850.038.02525342
AGAGCAC179900.037.7706838
CTCGTAT173750.037.76313444
CACATGT176850.037.61987331
ATGTCAG177550.037.4334434
TCGGAAG183500.037.179843
Produced by FastQC (version 0.11.2)