FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_vs6639-t3input-150818.351000000578ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_vs6639-t3input-150818.351000000578ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20820094
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG432760.207856890559668TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG60500.034.9579432
CGTCCGC61800.034.1497633
GCACACG65700.033.01315711
GATCGGA70450.031.6616041
CACGTCC68050.031.37692631
ACACGTC69850.030.72963313
AGTCACG69850.030.72941228
GTCCGCA68900.030.63070334
CCGCACA70000.029.98865336
CACGTCT72200.029.88510314
ACGTCTG72250.029.77099815
CACACGT73500.029.57094812
TCACGTC74300.028.67698330
TCCGCAC73500.028.65245835
CGTCTGA75300.028.50537516
AGCACAC78650.027.89237410
GTCACGT77050.027.77027329
CATCTCG77050.027.33271841
CAGTCAC80600.026.658827
GAGCACA82850.026.4240199
Produced by FastQC (version 0.11.2)