FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_vs6639-t3hop1-150818.35100000057784.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_vs6639-t3hop1-150818.35100000057784.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15867474
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1366450.8611641651342866TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG158850.042.02524611
GTCACGC158100.041.58340529
ACACGTC161900.041.20574613
CACACGT163650.040.84760312
AGTCACG162400.040.6209128
TCACGCC161800.040.54905730
GATCGGA167600.040.4223561
ACGTCTG165200.040.36900715
CACGTCT165800.040.141514
CACGCCA163650.039.98067531
AGCACAC169300.039.45795410
CGTCTGA169850.039.2505816
GAGCACA172300.038.9398359
CAGTCAC169700.038.86025627
GTATGCC170950.038.553845
TCGGAAG176700.038.3685463
TATCTCG171950.038.326239
TCCAGTC172300.038.2084525
AGAGCAC176100.038.2017758
CGTATGC172900.038.09187344
Produced by FastQC (version 0.11.2)