FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_mj-m31-160531.351000000579ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_mj-m31-160531.351000000579ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4407019
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG7500.023.39947511
ACACGTC8150.022.08539413
ACGTCTG8850.021.1012215
CACACGT8800.020.7097612
CATCTCG8250.020.4545539
ACTTAGG8700.020.1719632
CTTAGGC8750.019.54241833
CACGTCT9200.019.32021914
AGCACAC9750.018.23056610
GATCGGA9850.018.0534731
CGTCTGA10100.018.04415116
GAACTCC10500.016.92819221
CACTTAG11300.016.32706631
GAGCACA11150.016.1433189
CCAGTCA11100.016.01315526
GTATGCC11000.015.95527445
TTAGGCA11150.015.94134534
CTCGTAT11550.015.58459642
CAGTCAC11550.015.58406527
ATCTCGT11850.015.56962440