FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_mj-161-160531.35100000057d9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_mj-161-160531.35100000057d9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7478258
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG466910.624356634927546TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG56050.040.46346311
CACGAGT55450.039.72404531
ACGAGTG56250.039.07908232
AGTCACG57950.039.0586228
GATCGGA59050.038.8777121
GTCACGA57800.038.84856829
CACACGT58450.038.840512
ACGTCTG58600.038.66428415
CGAGTGG56800.038.66106433
ACACGTC58950.038.47289713
TCACGAG58650.038.40063530
CACGTCT60350.037.72952714
TATCTCG60800.037.0802241
CGTCTGA61050.037.0757916
AGCACAC61700.037.013410
GAGCACA62400.036.7779859
CAGTCAC62700.036.31493827
GAACTCC63500.035.96372221
CTCGTAT62750.035.8583744
TCCAGTC64050.035.58464425
Produced by FastQC (version 0.11.2)