FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_mj-157-160531.35100000057d21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_mj-157-160531.35100000057d21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19148468
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG310420.16211218568503757TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG45550.032.798811
CACGTGG48300.030.41848231
AGTCACG49200.029.99923528
ACACGTC50850.029.68997413
CACACGT51650.029.27367412
ACGTGGC50750.028.77266532
GATCGGA52050.028.7573051
TCACGTG52000.028.25408730
GTCACGT51750.028.04276529
CGTGGCC52200.027.88721833
CACGTCT55000.027.49056814
ACGTCTG55550.027.21831515
AGCACAC58600.025.72520410
CGTCTGA59250.025.670516
TATCTCG57900.025.64741741
GGCCTTA59550.024.52078636
GAACTCC62950.024.01845221
GAGCACA64750.023.7680459
CAGTCAC63350.023.7247327
TCCAGTC65700.022.8761325
Produced by FastQC (version 0.11.2)