FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_mj-117-160531.35100000057bd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_mj-117-160531.35100000057bd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7001742
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG7350.021.73414429
GCACACG8050.020.68285411
CACGGCT8800.018.664331
AGTCACG9250.017.75630828
TCACGGC9300.017.17698730
ACACGTC10150.016.40364513
CGGCTAC9800.015.61185233
CACACGT10650.015.42225612
GATCGGA11450.014.5462081
ACGGCTA10700.014.50898132
ACGTCTG11800.014.10991515
CATCTCG11400.013.81573739
CACGTCT11900.013.80227214
CGTCTGA12650.012.98395416
AGCACAC13250.012.7357110
GGCTACA13650.012.197494534
GAGCACA13950.012.0965559
TCCAGTC15750.011.14257625
CAGTCAC15900.010.75444627
CCAGTCA16150.010.72728426