FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_mj-113-160531.35100000057b66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_mj-113-160531.35100000057b66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8026769
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC120180.1497240047645572TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG17650.031.61375611
ACACGTC19100.029.44935213
CACACGT19500.028.61450212
GATCGGA21050.026.7325881
CACGTCT21200.026.42607114
ACGTCTG21250.026.25801515
CGTCTGA22350.024.96567216
TATCTCG22650.024.43694139
AGCACAC23700.023.73418410
CACTAGC24000.023.7180231
CAGTCAC24850.023.08781827
GAGCACA24800.023.0443619
GTATGCC24550.022.36462245
TCCAGTC25400.022.14498525
ATCTCGT24800.022.04637540
CGTATGC25550.022.01648344
GAACTCC25700.021.79893721
AGAGCAC26250.021.514298
CCAGTCA26350.021.3465926
CTCGTAT26250.021.08611342
Produced by FastQC (version 0.11.2)