FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_mj-109-160531.35100000057aee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_mj-109-160531.35100000057aee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9242087
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC117820.12748202868031863TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG19150.030.31280511
CGATCAG20900.027.23608833
ACACGTC21400.027.12571313
AGTCACG21350.027.08370628
GTCACGA22350.026.17391629
GATCGGA22500.026.008991
CACACGT22250.025.88720512
CACGTCT22550.025.74236114
CACGATC22550.025.5426731
ACGTCTG23050.025.18395815
GATCTCG24250.023.93774239
CGTCTGA24600.023.68862516
AGCACAC25100.023.12747610
GTATGCC25950.022.54480245
TCCAGTC27500.021.51758825
GAGCACA26850.021.4522699
CAGTCAC27750.021.08061227
CGTATGC28200.020.9049244
TCACGAT28700.020.46121830
CCAGTCA29200.020.033826
Produced by FastQC (version 0.11.2)