FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l07n01_mj-101-160531.35100000057a67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l07n01_mj-101-160531.35100000057a67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9976888
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG10400.012.5480411
CACACGT13750.010.47269612
GATCGGA13700.09.6934941
GTCACAC13300.09.30425229
ACACGTC13600.09.26467913
CACGTCT15000.08.39997614
ACGTCTG15050.08.37206915
CGTCTGA15350.08.35502516
GAGCACA17900.07.667469
AGCACAC17350.07.26233610
GTATGCC17700.07.1192345
CGTATGC19750.06.949694644
TCCAGTC19450.06.94064525
CAGTCAC18850.06.922883527
ATCTCGT20500.06.914475440
AGTCACA20400.06.72775328
GAACTCC20000.06.63728121
CTCGTAT20150.06.58826842
CCAGTCA21150.06.382826
TCACACT23500.06.223230430