FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6639-t6sir3-160326.35100000057982.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6639-t6sir3-160326.35100000057982.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23107880
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG2628421.1374561405027204TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC305500.042.41465413
CACGTCT307700.042.0382714
ACGTCTG308200.041.94798715
GATCGGA313100.041.8726161
AGCACAC312350.041.57073610
GAACTCC313100.041.125621
CGTCTGA314750.041.039316
GAGCACA317250.040.9496779
AGTTCCG308450.040.6887834
CCGTATC306050.040.53026238
CACACGT321600.040.38922512
TATCTCG306600.040.38469341
GTTCCGT309900.040.33193635
TCCAGTC318750.040.1778125
GTCACAG316050.040.0940829
CAGTCAC318850.040.02416627
CGTATCT309900.039.9982139
AGAGCAC326350.039.911248
AGTCACA319150.039.85964628
TCGGAAG331500.039.5897523
Produced by FastQC (version 0.11.2)