FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l06n01_vs6639-t6input-160326.35100000057b73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l06n01_vs6639-t6input-160326.35100000057b73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20600557
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG1043010.5063018441685824TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG129700.039.20551311
ACACGTC134200.037.7230313
AGTCACG131650.037.70163728
CACACGT135450.037.54128612
GATCGGA137700.037.4770851
ACGTCTG135350.037.4354915
CACGTCT137000.036.96838414
CGATCTC131300.036.50094240
CACGTGA137300.036.05212431
GTCACGT138200.035.67055529
CGTCTGA142500.035.63609716
TCACGTG138950.035.5268530
AGCACAC144150.035.3537410
GATCTCG139300.035.0992541
GAGCACA146400.034.7642069
GAACTCC145750.034.50176221
CAGTCAC144700.034.3790327
AGAGCAC151400.033.7052848
TCCAGTC148200.033.65828325
CCAGTCA148950.033.38306826
Produced by FastQC (version 0.11.2)